2018-09-01 · For cell differentiation, U937 cells (5 × 10 5 cells/well) were seeded onto 12-well plates and treated with 25 nM phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich) or 20 nM vitamin D 3 (VD3; Sigma-Aldrich), or co-stimulated with 25 nM PMA + 20 nM VD3 in RPMI 1640 medium supplemented with 0.5% FBS, 4 mM L-glutamine and 0.3% NaCO 3 and were incubated overnight at 37 °C with 5% CO 2 and then the cell cultures were supplemented with 5% FBS and incubated for 24 h to obtain PMA- or

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All these features can be used as robust markers for quality control of VD3- (VD3), is reported to alleviate excessive inflammatory response in many epithelial cells, including intestinal epithelial cells and keratinocytes.6,7 Although in clinical research, the beneficial effect of VD3 on periodontitis still remains to be confirmed; 8,9 reduced alveolar bone loss has been observed in murine experimental 1,25(OH) 2 D 3 (Sigma Aldrich, St. Louis, MO, USA) was reconstituted at 10 −4 M in 95% ethanol and stored at −20°C. The cells were grown in replicate using 1,25(OH) 2 D 3 as treatment and an equivalent concentration of 95% ethanol as vehicle (VHC). 1.3. ECM Glycoproteins and Coating Procedure LNCaP cells were sub-cultured 24 h before vitamin D treatment.

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Here we show that 1,25‐dihydroxyvitamin D3 (VD3, the active form of vitamin D) significantly promoted apoptosis in the undifferentiated gastric cancer cell line HGC‐27, and this was accompanied by a concurrent increase in phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression on VD3 treatment.

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17938 Sigma-Aldrich 25-Hydroxyvitamin D 3 monohydrate ≥99.0% (HPLC) Synonym: 25-Hydroxycholecalciferol CAS Number 63283-36-3. Empirical Formula (Hill Notation) C 27 H 44 O 2 · H 2 O . Molecular Weight 418.65 . Beilstein/REAXYS Number 4270041 . MDL number MFCD00867077. eCl@ss 34058003 .